We frequently receive very positive feedback on the speed and quality of
our Custom Antibody Services such as the following:
“ I've been trying to get the word out about your company within
[our institution]. They still cannot believe that we get the Abs so
quickly and at such a high titer.”
- Senior Researcher, leading U.S. university medical center, April 26, 2007
| Protein Polyclonal Antibody in 4 Weeks |
With Washington Biotechnology's ultra-fast 4-week proprietary protocol, your first lot of high titer antibody can be delivered to your lab just five weeks after immunization of two NZW Specific Pathogen-free (SPF) rabbits. We require 2-3 mg antigen for immunization and ELISA.
You receive:
120-140 ml antisera
60 - 70 ml shipped Week 5
60 - 70 ml shipped Week 7
1-2 ml preimmune serum from each animal
Antibody titers by ELISA on all collected sera
Optional Affinity Purification - You may chooses Affinity Purification as an option. We will send you a 6 - 10 ml sample of the First Production Bleed. If after your testing you decide to proceed, the additional charge is $ 300.00 for affinity purification of 120 - 140 ml of antisera.
In addition to our standardized immunization protocol which follows, customized protocols can also be developed, tailored to your research needs.
Rabbit Protocol
Our proprietary "WBI Immunization Protocol" for production of IgG antibody from rabbits takes 4 weeks.
This service includes:
- Animal Boarding and Care: 6 weeks
- Prebleed Serum: 2 ml
- 3 injections of protein/adjuvant emulsion
- Production Bleed at Week 4: 30 - 35 ml serum
Extended Antibody Production
Continued production of polyclonal antibody beyond the period of the WBI Immunization Protocol is an option available at our customer's choice. At the end of each immunization program, we hold the immunized animals for an additional 2 week period. During that period, you can notify us to continue production of antibody.
Prices for this extended production of antibody are as follow:
Rabbit Extended Antibody Production:
Includes: Collection of 60-70 ml antisera per rabbit per month, booster injections, antibody titers by ELISA and monthly maintenance.
| Price (USD) |
Shipping |
$200 per rabbit per month
(+ shipping) |
Shipping in North America is $75 USD
per one monthly shipment |
| Custom Monoclonal Antibody |
Our services for development of custom monoclonal antibody include all technical procedures from immunization of Balb/c mice to fusion and production of clones.
Our expertise includes optimal immunization protocols for Balb/c mice to achieve an effective antibody response.
Our WBI Standard Monoclonal Service gives you:
- Immunization of 4 Balb/c mice.
- Fusion of spleen cells with myeloma cells.
- ELISA screening for selection of positive hybridomas.
- Subcloning and expansion of selected hybridomas.
- Monoclonal antibody production of 5 selected clones in tissue culture.
| Breakdown of Standard Monoclonal Service by Phase: |
| Phase I |
- Mouse immunization and boosts (including adjuvants)
- ELISA evaluation of antibody titer prior to selection of mouse for fusion
Time: 6 weeks Price: $800 USD |
| Phase II |
- Mouse IV injections and hybridoma production (fusion, plating 6 X 96- wells, ELISA on well supernatants)
- Isolation and transfer of colonies secreting antibody for expansion.
- ELISA results
Time: 6-8 weeks Price: $3,000 USD |
| Phase III |
- Cloning of cells from selected supernatants by limiting dilution*
- Antibodies isotyped
- Clones expanded
- 5 vials (each vial >1 X 106 cells) frozen and shipped to client
- 10 ml supernatant from each clone shipped to client
Time: 8 weeks Price: $3,000 USD |
| Catalog No. |
Total Project Time |
Price |
| M-AB |
4-5 months |
$6800 USD |
|
Shipping |
$ 100 USD |
ORDER/REQUEST QUOTE |
Additional Services:
| * Additional clones (>5) in Phase III |
$300 USD per clone |
Purified MAb by Affinity Chromatography
from 1 liter tissue culture supernatant
(typical yield is 1 – 5 mg.) |
$500 USD |
Our client retains all property rights to cell lines and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality.
Your Resource for In Vivo Accuracy
