Rheumatoid Arthritis (RA) In Vivo Models: Collagen-induced Arthritis, Pristane-Induced Arthritis, Ovalbumin-induced Arthritis, Air Pouch , SCW PG-PS 10s


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WASHINGTON BIOTECHNOLOGY, INC.

Now in our 24th Year of Service in Drug Development

Rheumatoid Arthritis (RA) Animal Models

Drug & Vaccine Studies Drug Development Support Services Custom Antibodies

Arthritis Models Inflammation Xenograft Studies PK / TOX Rapid-turnaround

WBI’s models for Rheumatoid Arthritis (RA) cover all of the essential models for evaluation of anti-arthritic efficacy for small molecules, proteins and vaccines. Our scientists are experienced with evaluation of results for test materials and a wide variety of controls/reference materials used in hundreds of our prophylactic or therapeutic studies.

In addition to the basic read-outs for each model, optional analyses can be ordered for histopathology, cytokines, pharmacokinetic samples and collagen antibodies.

A Final Report for each of our studies includes all details of materials, procedures, observations, measurements, data and statistical analysis for treatment efficacy.

Overview of Washington Biotechnology RA Models

Collagen-Induced Arthritis (CIA)        The “standard” animal model for evaluation of anti-arthritic activity

                                                             - Model based on immunization with bovine collagen to develop antibodies against bone and cartilage

- 45-day study

- Species:     mouse or rat

- Prophylactic or Therapeutic

- Basic read-outs: Arthritic Index, body weights

- Optional:     Paw volumes, histopathology, cytokines, and collagen titers

Pristane-Induced Arthritis (PIA)         Non-immunogenic model that most closely simulates the autoimmune RA condition in humans

                                                           - 30-day study

- Well-defined inflammatory agent (pristane oil)

- Excellent model especially for the early stages of RA and hence for prophylactic or therapeutic intervention early in the disease

- Species:     rat

                                                          - Prophylactic or Therapeutic

- Readouts:   Arthritic score, body weights

- Optional:     Paw volumes, histopathology, cytokines, and collagen titers

Adjuvant-Induced Arthritis (AIA)       Economical in vivo screen; one of the longest established animal models for testing arthritis treatments

                                                            - 30-day study

- Reliable model with less well-defined mechanism than CIA or PIA

                                                           - Species:     rat

                                                           - Read-outs: Paw volumes, body weights



Ovalbumin-Induced Arthritis (OIA)     Advantage of synovial fluid measures at convenient volumes

                                                           - 30-60 day studies

                                                           - Species:     rabbit

                                                           - Read-outs: knee widths

                                                           - Optional services available as above

Air Pouch Model                                  Measurement of infiltrating cell types in response to inflammatory agent (carrageenan or other)

                                                                        - 6-day study

                                                            - Species:     mouse or rat

- The lining within the air pouch produced by injection of sterile air under the skin of the animal closely resembles the synovium in joints providing a convenient surface for study of the inflammatory cell response.

- Read-outs: CBC, cell differentials, exudate volumes and cytokines

PG-PS 10S Rat Model                        Allows evaluation of compound efficacy for acute or chronic (or both) inflammatory phases

                                                          - 22-day study

                                                            - Species:     rat

- An initial acute inflammatory response is seen within 3-6 days post-injection followed by a decline. A chronic response follows with a peak in measurements at approximately 12-16 days post-PG-PS 10S.

- Read-outs:   animal ankle widths