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WASHINGTON BIOTECHNOLOGY, INC.

Now in our 24th Year of Service in Drug Development

 

What's New

 

   

Drug & Vaccine Studies Drug Development Support Services Custom Antibodies
Arthritis Models Inflammation Xenograft Studies PK / TOX Rapid-turnaround

 

Anti-Cancer Drug Screening

We continue to expand our xenograft and orthotopic models for efficacy screening of drug candidates.

Our latest addition is a xenograft model in athymic nude mice for ovarian cancer – Human Cell Line OVCAR-5/RFP.

OVCAR-5 is a human epithelial carcinoma cell line of the ovary established from the ascetic fluid of a patient with progressive ovarian adenocarcinoma without prior cytotoxic treatment. The unique growth pattern of ovarian carcinoma makes it an ideal model for examining anticancer drug activity. In Vivo, OVCAR-5 cells can form moderately well-differentiated adenocarcinoma consistent with ovarian primary cells. The cell line stably expresses RFP and Puromycin resistant genes1.

WBI Cancer Models:

-        Cancers: brain, breast, colon, liver, peripheral blood (leukemia), lung, skin (melanoma), pancreas, prostate, ovarian, thyroid.

-        Animals: immunodeficient mice and rats.

-        Orthotopic: breast and liver cancers.

-        Tumor recoveries, preservation, weights, histology, metastasis observations.

Results:                Rapid, reliable, statistical analysis and complete Final Reports.

1.      Cell BioLabs, Inc. 2010-2011.

 

Indomethacin-Induced IBD Model

We have added a new model for Inflammatory Bowel Disease to our list of efficacy tests in this area.

In addition to in vivo models for TNBS-induced IBD and DSS-induced-IBD we now have available our validated Indomethacin-induced IBD model. Subcutaneous injection of Indomethacin to Wistar rats results in inflammation primarily of the small intestine. This model is also referred to as Small Intestine Injury. It is a valuable model for investigation of treatments for Crohn’s disease which mainly affects the last part of the Small Intestine (terminal ileum).

The Washington Biotechnology model provides a variety of readouts in a short (1-week) study.

-         Disease sign scores at termination: adhesions, ulceration and inflammation

-         Lengths of ulcerated and inflamed areas of the small intestine

-         Colon length and weight

-         Body weights daily

-         Collection and preservation of Small Intestine or sections (duodenum, jejunum, ileum)

-         Optional Items: 

 - Histopathology on all or part of the collected small intestine (scored readings,blocks, slides, photomicrographs)

 - Blood samples for exposure (plasma or serum)

Prophylactic or therapeutic dosing regimens to assess compound efficacy.

As with any of our in vivo models, each study protocol is custom designed to meet Sponsor requirements.  

References:

1.    Boushey RP et al. Glucagon-like peptide 2 decreases mortality and reduces the severity on indomethacin-induced enteritis. Am J Physiol Endocrinol Metab 277:E937-E947, 1999.

2.    Asano T et al. HSP70 Confers Protection against Indomethacin-Induced Lesions of the Small Intestine. J Pharmacol Exp Ther 330(2): 458-467, 2009.

3.    Yamada T et al. Mechanisms of acute and chronic intestinal inflammation induced by indomethacin. Inflammation 17: 641-662, 1993.

4.    Nygard GA et al. Acute indomethacin-induced jejunal injury in the rat: early morphological and biochemical changes. Gastroenterology 106:567-575, 1994.

 
 
 
Zymosan Air Pouch Model
 
WBI now offers the Zymosan Air Pouch Model.
 
Zymosan-A is a glucan with repeating glucose units connected by beta-1,3-glycosidic linkages. It is a particulate material prepared from Saccharomyces cerevisiae (baker’s yeast) cell wall and consists of protein-carbohydrate complexes.  Used as an inflammatory agent in the Air Pouch Model in mouse or rat, the combination has provided a useful in vivo method of assessing the anti-inflammatory activity of test materials. 
 
 
New Location
 
 
In January 2011 WBI moved to new and larger laboratory facilities in Baltimore, MD. Our address is:
 

Washington Biotechnology, Inc.
6200 Seaforth Street

Baltimore, MD 21224

USA

 
 
 
In Vitro Compound Screening  
 
 
To complement our In Vivo models, Washington Biotechnology offers In Vitro models for early screening of test materials
 
 
 

 

Body Conditioning Scoring for Rodents
 
 
 
This service is included without additional charge on all of our rodent studies extending a week or more.

This scoring is in addition to our standard observations regarding toxicity observations, if any, on our studies. And this does not replace reported toxicity effects in TOX studies.

We include this service to provide a useful evaluation of animal health status and to provide you with quality information throughout your study.

 

 

 

Scientific Meetings

Washington Biotechnology, Inc. regularly exhibits at major international scientific meetings.

 

Venues include:

American Association for Cancer Research 

 Inflammation Research Association

American Society for Cell Biology
American Society of Human Genetics
Biotechnica, Hannover, Germany
ENDO

Neuroscience

Experimental Biology

Institutional Vendor  Product Shows

 

USA - including UC San Diego, UCSF, UC Berkeley, UC Santa Cruz, Stanford University, Lawrence Livermore National Laboratory, Rocky Mountain National Laboratory, TSRI, University of Texas Medical Center, University of Chicago, University of Illinois, Duke University Medical Center, Resarch Triangle Park (RTP), University of Michigan, University of Washington, University of Pennsylvania, University of Nevada, Washington University St. Louis. VA Palo Alto (VAPA), Walter Reed Army Research Institute (WAIR) and Children's Hospital of Oakland Research Institute (CHORI).

Canada - including University of Alberta, University of Calgary, University of British Columbia, University of Western Ontario and University of Toronto.




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