Cholestasis, an impairment or cessation in the flow of bile, leads to hepatic and systemic accumulation of potentially toxic biliary compounds such as bile acids and bilirubin, resulting in acute liver toxicity, jaundice and hypercholesterolemia, and then aggravated outcomes just like hepatic fibrosis, cirrhosis and clinical signs of liver failure.

Alpha-naphthyl isothiocyanate (ANIT) is a hepatotoxicant used experimentally in rodents to model human intrahepatic cholestasis. Drug-induced liver injury and cholestasis in humans can be modeled by exposure of rodents to ANIT.

The purpose of this in vivo study is to provide information for development of human therapeutic products on acute cholestatic hepatitis. There is no in vitro model available to develop the information. Animal model is very popular for the study of acute cholestatic hepatitis to obtain the systemic information with the characteristics of acute cholestatic hepatitis.

ANIT-induced acute cholestatic hepatitis model in C57BL/6 mice

A rapid, robust and reliable assessment of treatments for acute cholestatic hepatitis

Model:

• Species: C57BL/6 mice, 7-9 weeks old, male
• Group size: 10
• Study Duration: one week
• Basic read-outs: AST, ALT, ALP, Total Bilirubin
• Optional: histopathology, cytokines

Liver chemistry function analysis post 48 hr oral administration of ANIT