Delayed-Type Hypersensitivity (DTH) Models
WBI has reliable Mouse Ear Edema models using a variety of topically-applied inflammatory agents principally oxazolone and DNFB (dinitrofluorobenzene) as the most widely used agents but extending to other inflammatory irritants.
Ear inflammation is rapidly produced leading to a 2-4 fold increase in ear volume within 24 hours, depending on the challenge agent used. The initial inflammatory response is due to rapid recruitment of macrophage and neutrophil cells followed 24-48 hours later by T-cell infiltration.
Also referred to as Delayed Type Hypersensitivity (DTH) and Contact Hypersensitivity models, these anti-inflammatory screens are used extensively at WBI with suitable controls (e.g. anti-IL17, betamethasone, prednisolone, celecoxib) for topical testing of anti-inflammatory agents. The models may also be used with equal reliability for non-topical, injections of test materials.
|Delayed-Type Hypersensitivity (DTH) Models|
To evaluate anti-inflammatory activity
(Oxazolone and DNFB mouse models)
|Study Duration||8 days|