A number of cell culture assays provide valuable insights into the efficacy of anti-inflammatory drugs in prevention of cytokine expression.  To complement our In Vivo models, WBI offers In Vitro models for early screening of test materials:

• IC50 Studies for cell inhibition efficacy

• Whole Blood Assay (WBA): LPS-stimulated human whole blood for anti-inflammatory screening of compounds in an environment simulating human circulation. Readouts: Cytokine measurements at various time periods to assess inflammatory response/inhibition, e.g. TNF-alpha, IL-1, IL-4, IL-6, IL-8, IL-10, IL-17, INF-gamma, COMP, other.

• PBMC Assay (human): Anti-inflammatory activity screening using purified PBMCs from selected human donors in a protein-free model. Readouts: Cytokine measurements as with Whole Blood Assay.

These services provide useful information for screening and selection of compounds for In Vivo testing.

As with our In Vivo studies, all of our In Vitro studies are carried out at our Maryland, USA facility by our full-time technical staff with an assigned Project Manager for your direct contact on any technical consultation.

Studies can usually be completed within 2 weeks of your order.